Evaluation of Antidiabetic Activity of Ethyl Linoleate Isolated from Decalepis hamiltonii Wight and Arn Seed

Aims: The main objective of the study was designed to screen the phytochemical constituents of the seed of Decalepis hamiltonii Wight & Arn and also to study its effect on blood glucose level of rats.

Study Design: Evaluation of Antidiabetic Activity of Ethyl Linoleate.

Place and Duration of Study: Department of Chemistry, Government Arts College (Autonomous), Kumbakonam, Tamilnadu State, India 612 001, between May 2014 and April 2015.

Methodology: The compound Ethyl Linoleate was isolated from Decalepis hamiltonii Wight and Arn seed by using Column chromatography. Structure of the compound was identified by GC-MS analysis, UV, IR, NMR spectroscopic methods and tested for alloxan induced diabetes in Male albino rats was evaluated.

Results: Biochemical parameters, including glucose (96.01+4.5), serum urea (26.01+0.01), creatinine (90.35+0.02), cholesterol (93.07+4.23) and protein (9.07+0.08), were also assessed. The isolated Ethyl Linoleate increased by body weight (200±1.32) and significantly reduced the glucose level (96.01+4.5) at 20 mg/kg was almost equally the reference standard, glibenclamide. The Ethyl Linoleate enhance insulin release from the pancreatic β cells which is glucose dependent and there by decrease the blood glucose level in alloxan induced diabetic rats. The Ethyl Linoleate enhance insulin release from the pancreatic β cells which is glucose dependent and there by decrease the blood glucose level in alloxan induced diabetic rats. Further biochemical investigations will clearly elucidate the mechanism of action and will be helpful this compound as a therapeutic target in diabetes research. The histopathological studies of the rat pancreas showed recovery of the alloxan induced damage of the insulin secreting β pancreatic cells, thus providing a hint for the mechanism of the test drug.

Conclusion: The compound Ethyl Linoleate was isolated from Decalepis hamiltonii Wight and Arn seed by using Column chromatography for the first time. The investigation of the mechanisms of this drug may contribute for the development of new drugs for the treatment of diabetes.