Evaluation of the Organ Microscopy, Chemical Composition and Anti-inflammatory Potentials of Methanolic Leaf Extract and Fractions of Diaphananthe bidens

Umeyor, Immaculeta C. and Umeyor, Chukwuebuka E. and Ogbue, Cyril O. and Onyegbule, Felix A. and Okoye, Festus B. C. (2021) Evaluation of the Organ Microscopy, Chemical Composition and Anti-inflammatory Potentials of Methanolic Leaf Extract and Fractions of Diaphananthe bidens. Asian Plant Research Journal, 8 (1). pp. 16-28. ISSN 2581-9992

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Abstract

Aim: The study aims to investigate high-pressure liquid chromatographic (HPLC)-based chemical composition and anti-inflammatory activity of methanolic leaf extract and fractions of D. bidens.

Study Design: The study is a laboratory-based research involving collection, identification and preparation, of plants, extraction, characterization and evaluation of the anti-inflammatory potential of D. bidens.

Place and Duration of Study: Department of Pharmacognosy and Traditional Medicine, Faculty of Pharmaceutical Sciences, Nnamdi Azikiwe University, Awka, Nigeria between January 2016 and June 2019.

Methodology: Powdered leaves of D. bidens was macerated in methanol and the obtained crude extract was fractionated using n-hexane, ethylacetate, butanol, and water respectively. The acute toxicity profile of the extract was determined. Spectroscopic factors of the plant were assayed using HPLC-DAD and the anti-inflammatory activity of the extract and its fractions were evaluated.

Results: Acute toxicity study showed that the crude extract has an LD50 > 5,000 mg/kg. HPLC-DAD analysis revealed the presence of compounds including apigenin monoglycoside, quercetin 3-O-(6''O-acetyl) galactopyranoside, kaempferol 3-O-(6" O-malonyl) glucoside, genistein 8-C-glucoside, while anti-inflammatory study showed that the extract and fractions inhibited cellular activities of inflammatory mediators (86 – 97 %) in comparison with the diclofenac (53 %); thus, supporting its ethnomedicinal use in the treatment of inflammation.

Conclusions: Methanolic extract of D. bidens has wide margin of safety. It has sufficient deposit of flavonoids which might be responsible for its strong anti-inflammatory activity that is comparable with diclofenac.

Item Type: Article
Subjects: Open Archive Press > Agricultural and Food Science
Depositing User: Unnamed user with email support@openarchivepress.com
Date Deposited: 03 Mar 2023 07:58
Last Modified: 29 Jul 2024 08:22
URI: http://library.2pressrelease.co.in/id/eprint/196

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