Abdul Rahim, Rohanizah and Ahmad, Nor Hazwani and Al Azzam, Khaldun Mohammad and Mat, Ishak (2018) Determination and Quantification of the Vinblastine Content in Purple, Red, and White Catharanthus Roseus Leaves Using RP-HPLC Method. Advanced Pharmaceutical Bulletin, 8 (1). pp. 157-161. ISSN 2228-5881
apb-8-157.pdf - Published Version
Download (699kB)
Abstract
Purpose: To determine and quantify vinblastine in different varieties of Catharanthus roseus using reversed-phase HPLC method. Methods: The liquid chromatographic separation was performed using a reversed phase C18, Microsorb - MV column (250 mm x 4.6 mm, 5 µm) at room temperature and eluted with a mobile phase containing methanol – phosphate buffer (5 mM, pH 6.0) – acetonitrile with different proportion gradient elution at a flow rate of 2.0 mL min-1 and detection at 254 nm. Results: The HPLC method was utilized for the quantification of vinblastine in purple, red and white varieties of Catharanthus roseus leaves. The separation was achieved in less than 8 min. The peak confirmation was done based on the retention times and UV spectra of the reference substance. The method was validated with respect to linearity, precision, recovery, limit of detection and quantification. Results showed that the purple variety gives 1.2 and 1.5 times more vinblastine concentration compared to the white and pink varieties, respectively. Conclusion: The obtained results from different varieties are thus useful for the purpose of vinblastine production from Catharanthus roseus plant.
Item Type: | Article |
---|---|
Subjects: | Open Archive Press > Medical Science |
Depositing User: | Unnamed user with email support@openarchivepress.com |
Date Deposited: | 14 Apr 2023 05:40 |
Last Modified: | 03 Oct 2024 04:49 |
URI: | http://library.2pressrelease.co.in/id/eprint/942 |