Analysis of Genetic Diversity in Maize (Zea mays L.) Variety Using SSR Markers

The study was carried out to analyze the genetic diversity of five maize varieties at K. K. Wagh collage of agricultural Biotechnology, Nashik during May to October 2022 Genomic DNA was isolated from maize varieties by using CTAB method. The PCR amplification obtains with set of five SSR primers. The dendrogram was constructed based UPGMA method from the Jaccard’s similarity coefficient and the varieties subjected to cluster analysis. Among the five SSR primer used only three found polymorphic with total 13 alleles. The PIC value 0.358 to 0.868 of Phi-085, ssr-08 and ssr-06 shows polymorphism 50%, 80% and 83.34% respectively. The cluster analysis indicates the varieties were divided into 3 clades and one Simplicifolicus. Interpreting the results P. madhu is divers among the all varieties. The cluster analysis indicates the P. madhu variety shows the diversity. The genetic diversity of maize will help the breeder to select the parents for different breeding programs.